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. 2015 Sep 17;35(20):3528–3546. doi: 10.1128/MCB.00518-15

Fig 1.

Fig 1

Vav family TKO mice show protection against heart hypertrophy and cardiorenal fibrosis. (A) Evolution of mean arterial pressure in mice of the indicated genotypes. *, P ≤ 0.05; ***, P ≤ 0.001 (n = 8). (B) Examples of aorta, heart ventricle, left ventricle, and kidney sections (from left to right) obtained from 4-month-old mice of the indicated genotypes. Sections were stained as indicated. Sirius red-stained fibrotic deposits in interstitial areas are seen as dark pink areas (right). amw, aorta media wall; lv, left ventricle; rv, right ventricle. Bars, 100 μm. Similar results were obtained using serial sections and independent mice (n = 4). (C to F) Status of cardiovascular (C and D) and cardiorenal (E and F) parameters in mice of the indicated genotypes. LV, left ventricle. Asterisks indicate significant differences (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001) from the control or between the experimental groups indicated (n = 4). (G) Examples of kidney sections from mice of the indicated genotypes. WT mice exhibit normal glomeruli (g) and tubules (t), whereas DKO animals display typical signs of glomerular sclerosis, cytoplasmic vacuolar accumulation (v) in necrotic tubules, and protein casts (c). Erythrocytes (e) are seen as red spots in all sections. Bars, 50 μm. (H to J) Daily urine production (n = 6) (H), urine protein content (n = 6) (I), and creatinine clearance rates (n = 4) (J) in mice of the indicated genotypes. An asterisk indicates a significant difference (P ≤ 0.05) from the control or between the experimental groups indicated. (K) Levels of Lcn2 transcripts in the kidneys of animals of the indicated genotypes. An asterisk indicates a significant difference (*, P ≤ 0.05) from the control or between the experimental groups indicated (n = 4). (L to N) AUC (area under the curve) for mean arterial pressure (L) and renal parameters (M and N) in mice of the indicated genotypes that had either been left untreated (−) or infused with AngII (+). Asterisks indicate significant differences (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001) from the control or between the experimental groups indicated (n = 4). NS, not statistically significant. Error bars in all figures represent the standard errors of the means.