FIG 5.

Immunodepletion of T_REG cells restores renal fibrosis in Vav1-deficient mice. (A) Evolution of the CD4⁺ CD25⁺ cell population in Vav1^−/− mice upon immunodepletion with antibodies to CD25. The time of injection (t₀) is indicated by an arrow. ***, P ≤ 0.001 (n = 4). (B to D) Cardiorenal fibrosis levels (B and C) and AUC for mean arterial pressure (D) in Vav1^−/− mice treated as indicated. Asterisks indicate significant differences (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001) from the control or between the indicated experimental groups (n = 4). (E and F) Representative example (E) and quantification (F) of neutrophils in kidneys from Vav1^−/− mice used in the experiments for which results are shown in panels A to D. Asterisks indicate significant differences (**, P ≤ 0.01) from the control or between the indicated experimental groups (n = 4). (G) Quantification of kidney-resident neutrophils in Vav1^−/− mice under the indicated conditions. *, P ≤ 0.05 (n = 4).