FIG 6.

Neutrophils are involved in AngII-driven cardiorenal fibrosis. (A) Impact of the immunodepletion step on the numbers of neutrophils in WT mice. ***, P ≤ 0.001 (n = 4). The time points of antibody injection (α-Ly6G) and implantation of osmotic pumps (AngII) are indicated by arrows. (B and C) Extent of cardiorenal fibrosis obtained in WT animals injected with either a control antibody or an anti-Ly6G antibody and subsequently infused with AngII as indicated. Asterisks indicate significant differences (*, P ≤ 0.05) from the control or between the indicated experimental groups (n = 4). (D) AUC for the mean arterial pressure increase obtained in the experiment for which results are shown in panels A to C. Asterisks indicate significant differences (*, P ≤ 0.05; ***, P ≤ 0.001) from the control or between the indicated experimental groups (n = 4). (E and F) Quantification of kidney-resident macrophages (E) and CD4⁺ T cells (F) at the end of these experiments. (G and H) Representative dot plots (G) and quantification (H) of neutrophils present in kidneys at the end of these experiments. Asterisks indicate significant differences (**, P ≤ 0.01) from the control or between the indicated experimental groups (n = 4).