TABLE 1.
Percentages of hematopoietic subpopulations in mice of various genotypes
| Tissue and cell type | % of total hematopoietic cells in mice of genotypea: |
|||
|---|---|---|---|---|
| WT | DKO | TKO | Vav1−/− | |
| Kidney | ||||
| CD11b+ Gr1+ neutrophils | 0.37 ± 0.07 | 0.91 ± 0.17* | 0.1 ± 0.03*† | 0.15 ± 0.01*† |
| CD4+ T cells | 0.14 ± 0.02 | 0.13 ± 0.01 | 0.02 ± 0.005*† | 0.02 ± 0.000*† |
| CD4+ Foxp3+ TREG cells | 0.06 ± 0.03 | 0.07 ± 0.02 | 0.02 ± 0.003 | 0.01 ± 0.002 |
| CD11b+ F4/80+ macrophages | 0.69 ± 0.16 | 0.73 ± 0.11 | 0.45 ± 0.07 | ND |
| CD11c+ dendritic cells | 0.26 ± 0.04 | 0.26 ± 0.04 | 0.3 ± 0.03 | ND |
| CD11c− dendritic cells | 0.16 ± 0.04 | 0.17 ± 0.05 | 0.14 ± 0.03 | ND |
| CD8+ T cells | 0.60 ± 0.06 | 0.68 ± 0.047 | 0.643 ± 0.031 | ND |
| B220+ B cells | 18.80 ± 1.09 | 18.98 ± 1.75 | 18.467 ± 2.17 | ND |
| Blood | ||||
| CD11b+ Gr1+ neutrophils | 18.30 ± 2.6 | 48.20 ± 2.60* | 46.3 ± 7*† | 12.1 ± 1.3 |
| CD4+ T cells | 22.08 ± 2.11 | 29.24 ± 3.10 | 7.43 ± 0.14*† | 5.73 ± 0.21*† |
| CD4+ Foxp3+ TREG cells | 1.22 ± 0.21 | 2.46 ± 0.36 | 1.63 | 1.35 ± 0.04 |
| CD8+ T cells | 16.07 ± 0.91 | 7.67 ± 1.94 | 4.03 ± 1.35*† | 6.09 ± 0.72*† |
| B220+ B cells | 29.25 ± 9.33 | 12.24 ± 3.65* | 7.92 ± 2.01* | 7.34 ± 2.96* |
| Spleen | ||||
| CD4+ T cells | 10.07 ± 0.48 | 12.17 ± 1.67 | 3.77 ± 0.44*† | 4.35 ± 0.32*† |
| CD4+ Foxp3+ TREG cells | 1.04 ± 0.065 | 0.95 ± 0.02 | 0.91 ± 0.02 | 1.11 ± 0.079 |
| CD8+ T cells | 10.60 ± 0.69 | 8.52 ± 0.37 | 3.43 ± 0.49*† | 3.41 ± 0.17*† |
| B220+ B cells | 70.25 ± 1.19 | 71.4 ± 1.9 | 33.7 ± 2.84*† | 26.2 ± 3.08*† |
| Thymus | ||||
| CD4+ CD8− T cells | 9.71 ± 0.53 | 9.34 ± 0.64 | 2.77 ± 0.25*† | 5.21 ± 1.07*† |
| CD4+ Foxp3+ TREG cells | 0.49 ± 0.04 | 0.39 ± 0.06 | 0.29 ± 0.06 | 0.6 ± 0.18 |
| CD4− CD8+ T cells | 6.57 ± 2.03 | 2.93 ± 0.32 | 1.8 ± 0.19*† | 3.39 ± 0.68*† |
| CD4+ CD8+ thymocytes | 75.3 ± 1.96 | 80.5 ± 1 | 40.23 ± 1.52*† | 59.3 ± 5.57*† |
| CD4− CD8− thymocytes | 8.24 ± 0.86 | 7.24 ± 0.3 | 55.17 ± 1.52*† | 31.6 ± 5.16*† |
a
The percentages of the indicated cells in the total population analyzed were determined by flow cytometry (see Materials and Methods). Results are means ± standard deviations for six mice per genotype. Values that are statistically significantly different from those for WT mice are shown in boldface. Asterisks and daggers indicate significant differences (P ≤ 0.05) from results for WT and DKO mice, respectively. ND, not determined.