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. 2015 Sep 17;35(20):3448–3458. doi: 10.1128/MCB.00189-15

FIG 4.

FIG 4

Mapping of the cis-acting determinants of protection. A series of SPS4 (yellow, with the 5′ UTR indicated in orange)-SPO20 (blue, with the 5′ UTR indicated in green) chimeric genes were constructed in the NDT80-IN background (A14201). Sequence swaps were made at the translational start site (AUG) or the reported transcriptional start sites (indicated by the red arrows). (A) SPS4. (B) SPO20. (C) SPS4 upstream region fused to the SPO20 coding region. (D) SPO20 upstream region fused to the SPS4 coding region. (E) SPS4 promoter fused to the SPO20 5′ UTR and coding region. (F) SPO20 promoter fused to the SPS4 5′ UTR and coding region. (G) SPO20 promoter and coding region with the SPS4 5′ UTR. (H) Transcript levels before and after return to growth were assayed for each construct. The values for bars A and B are from Fig. 3. The dashed line indicates a ratio of 1. The error bars represent the range of values from the two experiments.