FIG 6.

Commitment to meiosis in wild-type and ime2ΔC241-as cells. (A) (Top) At the indicated times after estradiol addition, commitment was analyzed by transfer of cells to rich medium and determination of the budding index using light microscopy. For NDT80-IN cells (A14201), the percentage of budding cells was determined after 3 h of incubation in rich medium. (Bottom) Progression of the population through the meiotic divisions in the same time course. The percentages of cells containing one nucleus (before MI), two nuclei (MI), or four nuclei (MII or later) were determined by DAPI staining. (B) Commitment monitored in NDT80-IN ime2ΔC241-as cells (yLJ92). The cells were treated as for panel A, except that 50 μM 3-MB-PP1 was added to the rich medium upon return to growth in order to inactivate Ime2-as.