TABLE 1.
Strains used in this study
| Strain name | Genotype | Reference |
|---|---|---|
| AN120 | MATa/MATα ura3/ura3 his3ΔSK/his3ΔSK trp1::hisG/trp1::hisG arg4-NSP1/ARG1 lys2/lys2 hoΔ::LYS2/hoΔ::LYS2 rme1Δ::LEU2/RME1 leu2/leu2 | 36 |
| AN117-4B | MATα ura3 his3 trp1::hisG leu2 arg4-NSP1 lys2 hoΔ::LYS2 rme1Δ::LEU2 | 36 |
| A14201 | MATa/MATα hoΔ::LYS2/hoΔ::LYS2 lys2/lys2 ura3/ura3 leu2::hisG/leu2::hisG his3::hisG/his3::hisG trp1::hisG/trp1::hisG GAL-NDT80::TRP1/GAL-NDT80::TRP1 ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 | 14 |
| A31421 | MATa/MATα ho::LYS2/ho::LYS2 lys2/lys2 ura3/ura3 leu2::hisG/leu2::hisG his3::hisG/his3::hisG trp1::hisG/trp1::hisG GAL-NDT80::TRP1/GAL-NDT80::TRP1 ura3::pGPD1-GAL4(848).ER::URA3/ura3::pGPD1-GAL4(848).ER::URA3 CLB3-3HA::Kan/CLB3-3HA::Kan rim4F349L-3V5/rim4F349L-3V5 | 15 |
| yLJ28 | As A14201 plus HIS3MX6-PSPS4-5′ UTRSPS4-SPO20/HIS3MX6-PSPS4-5′ UTRSPS4-SPO20 | This study |
| yLJ29 | As A14201 plus HIS3MX6-PSPS4-5′ UTRSPO20-SPO20/HIS3MX6-PSPS4-5′ UTRSPO20-SPO20 | This study |
| yLJ40 | As A14201 plus HIS3MX6-PSPO20-5′ UTRSPS4-SPS4/HIS3MX6-PSPO20-5′ UTRSPS4-SPS4 | This study |
| yLJ41 | As A14201 plus HIS3MX6-PSPO20-5′ UTRSPO20-SPS4/HIS3MX6-PSPO20-5′ UTRSPO20-SPS4 | This study |
| yLJ44 | As A14201 plus KanMX6-PSPO20-5′ UTRSPS4-SPO20/KanMX6-PSPO20-5′ UTRSPS4-SPO20 | This study |
| yLJ50 | As A14201 plus IME2ΔC241::HIS3MX6/IME2ΔC241::HIS3MX6 | This study |
| yLJ80 | As A14201 plus SPS4-3HA-HIS3MX6/SPS4-3HA-HIS3MX6 | This study |
| yLJ89 | As A14201 plus IME2ΔC241::HIS3MX6/IM2ΔC241::HIS3MX6 SPS4-3HA-KanMX/SPS4-3HA-KanMX | This study |
| yLJ92 | As A14201 plus IME2ΔC241(M146G)-as/IME2ΔC241(M146G)-as | This study |
| yJL97 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SPS4-12×MS2L | This study |
| yLJ99 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPS4-12×MS2L/SPS4 | This study |
| yLJ111 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPR6-12×MS2L/SPR6 | This study |
| yLJ112 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 LDS2-12×MS2L/LDS2 | This study |
| yLJ113 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPS1-12×MS2L/SPS1 | This study |
| yLJ119 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SGA1-12×MS2L/SGA1 | This study |
| yLJ120 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 CTS2-12×MS2L/CTS2 | This study |
| yLJ121 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPO20-12×MS2L/SPO20 | This study |
| yLJ122 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPR28-12×MS2L/SPR28 | This study |
| yLJ123 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPS4-12×MS2L/SPS4 IME2ΔC241::KanMX6/IME2ΔC241::KanMX6 | This study |
| yLJ137 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPO77-12×MS2L/SPO77 | This study |
| yLJ139 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SSP2-12×MS2L/SSP2 | This study |
| yLJ141 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 CDA1-12×MS2L/CDA1 | This study |
| yLJ159 | MATa/MATα his3Δ1/his3 leu2Δ0/leu2 met15Δ0/MET15 ura3Δ0/ura3 trp1::hisG/TRP1 arg4-NSP1/ARG4 lys2/LYS2 ho::LYS2/ho rme1::LEU2/RME1 SPS4-12×MS2L/SPS4 rim4-F349L/rim4-F349L | This study |
| yLJ167 | As A31421 plus SPS4-GFP-HIS3MX6/SPS4-GFP-HIS3MX6 | This study |