TABLE 1.
Bacillus subtilis strains used in this study
| Strain | Genotypea | Sourceb or reference |
|---|---|---|
| 168 | trpC2 | Laboratory collection |
| BKE25330 | ΔpgpH::ermC | Bacillus Genetic Stock Center (BGSC) |
| GP588 | trpC2 ΔglmM::spc; complemented with pGP400 | 33 |
| GP983 | trpC2 ΔcdaS::ermC | 7 |
| GP985 | trpC2 ΔcdaAR::cat | 7 |
| GP987 | trpC2 ΔdisA::tet | 7 |
| GP991 | trpC2 ΔcdaS::ermC ΔdisA::tet | 7 |
| GP997 | trpC2 ΔcdaA::cat | 7 |
| GP998 | trpC2 ΔgdpP::spc | 7 |
| GP999 | trpC2 ΔcdaR::cat | 7 |
| GP1320 | trpC2 ganA::(pxylA glmMBsu aphA3) | pGP2572 → 168 |
| GP1321 | trpC2 ganA::(pxylA glmMBsu aphA3) ΔdisA::tet ΔcdaS::ermC | GP1320 → GP991 |
| GP1327 | trpC2 ganA::(pxylA cdaS aphA3) ΔcdaS::ermC ΔdisA::tet ΔcdaR::cat | 7 |
| GP1331 | trpC2 ganA::(cdaR-Strep tag aphA3) cdaA-3×FLAG ermC | 7 |
| GP1365 | trpC2 ganA::(pxylA glmMEco aphA3) | pGP2566 → 168 |
| GP1367 | trpC2 ganA::(pxylA glmMEco aphA3) ΔglmM::spc | GP588 → GP1365 |
| GP1374 | trpC2 ganA::(pxylA glmMEco aphA3) ΔglmM::spc ΔcdaS::ermC ΔdisA::tet | GP983 + GP987 → GP1367 |
| GP1376 | trpC2 ΔgdpP::spc ΔcdaA::cat | GP997 → GP998 |
| GP1381 | trpC2 cdaA-3×FLAG ermC | pGP1966 → 168 |
| GP1383 | trpC2 ganA::(pxylA glmMBsu aphA3) ΔglmM::spc ΔcdaS::ermC ΔdisA::tet | GP588 → GP1321 |
| GP2032 | trpC2 ΔcdaS::ermC ΔcdaA::cat | GP997 → GP983 |
| GP2034 | trpC2 ΔpgpH::ermC | BKE25330 → 168 |
| GP2035 | trpC2 ΔgdpP::spc ΔpgpH::ermC ΔdisA::tet | GP987 → GP2040 |
| GP2040 | trpC2 ΔgdpP::spc ΔpgpH::ermC | BKE25330 → GP998 |
| GP2041 | trpC2 ΔgdpP::spc ΔcdaA::cat ΔpgpH::ermC | BKE25330 → GP1376 |
| GP2042 | trpC2 ΔgdpP::spc ΔpgpH::ermC cdaA1–795 ΔcdaR::cat | GP999 → GP2040 (suppressor) |
| GP2051 | trpC2 ΔgdpP::spc ΔpgpH::ermC ΔcdaAR::cat | GP985 → GP2040 |
| GP2064 | trpC2 ΔgdpP::spc ΔpgpH::ermC cdaA-ins295 | Suppressor of GP2040 |
| GP2065 | trpC2 ΔgdpP::spc ΔpgpH::ermC cdaA-del381 | Suppressor of GP2040 |
| GP2134 | trpC2 ΔpgpH::ermC ΔdisA::tet ΔgdpP::spc cdaASD-mut | Suppressor of GP2035 |
a
glmMBsu, B. subtilis glmM gene; glmMEco, E. coli glmM gene; cdaA1-795, truncated cdaA gene; cdaA-ins295, cdaA with insertion after base 285; cdaA-del381, cdaA with deletion of base 382; cdaASD-mut, cdaA with mutated Shine-Dalgarno sequence.
b
Arrows indicate construction by transformation.