FIG 1.

Western blot of purified envelopes. Envelopes were purified from overnight cultures of a ΔacrAB strain containing either the empty vector plasmid pACYC184 (first lane) or plasmid clones expressing various AcrB proteins, as indicated. Detergent-solubilized envelope samples were analyzed by SDS-PAGE and electrotransferred to PVDF membranes. Membranes were blotted with primary antibodies against AcrB-MBP. LC, nonspecific band used as a gel loading control. AcrB levels were determined relative to those of LC and then normalized to the wild-type value of 1.