FIG 7.

Relationship between the carbon source and rpoS. (A) Effects of the carbon sources on the transformation ability of the ΔrpoS mutant. The ΔrpoS mutant was incubated in LB broth with various carbon sources (0.2%), and transformation experiments were performed as described in the legend to Fig. 1. (B) RpoS protein levels of BW25113 cultivated in LB medium with various carbon sources (0.2%) added. Strains were grown to log phase (OD₆₀₀ of 2.0) and analyzed by immunoblotting as described in Materials and Methods.