TABLE 2.
Oligonucleotides used in this study
| Purpose and primer | Sequencea |
|---|---|
| Construction of cloned genes in plasmids | |
| crp F | 5′-GGTCTAGACTTCACTCGCGCTTGCATTT-3′ |
| crp R | 5′-AGGGATCCCACTCCGACGGGATTAA-3′ |
| lacZ F | 5′-GGAAGCTTATGACCATGATTACGGAT-3′ |
| lacZ R | 5′-GATCTAGATTATTTTTGACACCAG-3′ |
| Construction of rpoS with site-directed mutagenesis | |
| rpoS−165 F | 5′-CGGGATCCGAACGTTGGTCAG-3′ |
| rpoS+1712 R | 5′-GCTCTAGACGACCATTCTCGG-3′ |
| rpoS+570 R | 5′-CCCAAGCTTCATAAGGTGGC-3′ |
| rpoS M1 R | 5′-TGCAGGCTTGATTATGACTCCTTGC-3′ |
| rpoS M2 R | 5′-GGCGCGTTTGCCGTGGCTGTGGTTGG-3′ |
| Construction of rpoS knockout mutant | |
| rpoS H1P1 | 5′-TGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCTGTAGGCTGGAGCTGCTTCG-3′ |
| rpoS H2P2 | 5′-TTTTTGACGAAAAGGCCTTAGTAGAATAGGAACCCAGTGATAACGATTTGATTCCGGGGATCCGTCGACC-3′ |
a
Modified nucleotides for site-directed mutagenesis are in boldface.