(A) Graph showing expression of p63 (x) and Slug (y) in breast tumors. (B) Immunoblots showing expression of Slug and ΔNp63α in cell lines transfected as indicated (n=2). (C) Wound healing of MCFDCIS cells transfected as indicated. Scale bars, 1 mm. Graph shows relative wound area (mean + SD, n=6 wounds from 2 independent experiments). ****p< 0.0001, unpaired Student's t-test. (D) MCFDCIS cells transfected as indicated were wounded and imaged for 7 h. Scale bars, 100 μm. Graphs show cell speed and displacement (mean ± SD, n=10 x,y positions over 2 independent experiments). ****p< 0.0001, unpaired Student's t-test. (E) Wound healing of MCFDCIS and MCFDCIS-Slug cells transfected as indicated. Scale bars, 1 mm. Graph shows quantification of wound area (mean + SD, n=9 wounds from 3 independent experiments). ****p< 0.0001, unpaired Student's t-test. (F) Graphic showing ΔNp63α regulation of Slug and miR205.