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. 2015 Sep 1;29(17):1801–1816. doi: 10.1101/gad.266999.115

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© 2015 Arenzana et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — T-cell differentiation in Ikzf1^ΔF4/ΔF4 mice progresses through a CD4⁺ ISP stage instead of a CD8⁺ ISP stage. (A) Shown are staining profiles of thymocytes in wild-type (WT) and Ikzf1^ΔF4/ΔF4 mice at 4, 5, and 6 wk of age. The absolute mean percentages are indicated (n = 5–8). (B) Shown are absolute numbers of the indicated thymocyte populations in wild-type and Ikzf1^ΔF4/ΔF4 mice at 4 wk (n = 5–8), 5 wk (n = 6–7), and 6 wk (n = 5–10). SP cells are gated on TCRβ^hi. (C) Shown are staining profiles of total (left), CD8⁺ gated (middle), and CD4⁺ gated (right) thymocytes of 4-wk-old wild-type and Ikzf1^ΔF4/ΔF4 mice. The absolute percentages indicated are representative of five to eight mice. (D) Shown are staining profiles of Annexin V (AnnV) and 7-AAD (DNA content) for wild-type and Ikzf1^ΔF4/ΔF4 TCRβ^lo CD4⁺ ISP thymocytes; the fractions of AnnV⁻ 7-AAD⁻ viable cells, AnnV⁺ 7-AAD⁻ early apoptotic cells, and AnnV⁺ 7-AAD⁺ dead cells are indicated. Data are representative of three independent experiments. (E) Shown are cell cycle analyses of TCRβ^lo CD8⁺ gated (left) and TCRβ^lo CD4⁺ gated (right) cells using BrdU and 7-AAD (DNA content) staining, with cells in the G0/G1, S, and G2/M phases gated. Percentages are representative of three independent experiments.