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. 2015 Sep 1;29(17):1875–1889. doi: 10.1101/gad.264127.115

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© 2015 Nicolay et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Loss of RB1 leads to decreased respiration and mitochondria activity. (A–C) Loss of pRb decreases OCR independently of mitochondrial mass differences. (B,C) RB^KO RPE cells. n = 14, repeated twice. (D) Loss of pRB in RPE cells caused little to no difference in 2-NDBG uptake. (E) Extracellular acidification rate (ECAR) measurement in response to glucose challenge. (F–H) OCR measurements in response to glucose (F), glutamine (G), or glutamine + pyruvate (H). For the ECAR and OCR analysis in E–H, n = 14 replicates, repeated twice. (I) The respiratory control ratio is decreased in RB^KO RPE cells. n = 14 replicates, repeated twice. Error bars are the 95% confidence intervals. Statistical significances are as follows: (*) P < 0.05; (**) P < 0.02; (***) P < 0.001. Statistical differences are between the effects from RB^−/− compared with control cells.