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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Leuk Res. 2015 Jul 2;39(10):1071–1078. doi: 10.1016/j.leukres.2015.06.009

Fig. 1.

Fig. 1

The effect of 4-HPR on ceramide levels and viability in human AML cell lines. A. Effect of 4-HPR on ceramide levels. Cultures without and with 4-HPR (5.0 μM) in the medium were radiolabeled by addition of [3H]palmitic acid for 24 h, and total lipids were extracted and analyzed by TLC as detailed in Materials and methods. B. Effect of 4-HPR on cell viability. Cultures were exposed to 4-HPR at the concentrations indicated for 72 h, after which viability was measured as described in Materials and methods. Data shown (n=6 for each point) represent the mean ± S.E. Triplicate experiments yielded similar results.