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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Leuk Res. 2015 Jul 2;39(10):1071–1078. doi: 10.1016/j.leukres.2015.06.009

Fig. 4.

Fig. 4

The effect of 4-HPR on ROS generation and vitamin E on 4-HPR-induced cytotoxicity. A. Effect of 4-HPR on ROS generation in HL-60/VCR cells. Cells were seeded in 6-well plates and exposed to 4-HPR at the concentrations shown for 24 h. ROS was measured by fluorescence detection using a cell-permeable probe as detailed in Materials and methods. B. Effect of vitamin E on 4-HPR-induced cytotoxicity in HL-60/VCR and KG-1 cells. HL-60/VCR and KG-1 cells (20,000 cells/well, 96-well plate) were exposed to 4-HPR and/or vitamin E at the micromolar concentrations shown in parentheses. After 24 h, viability was assessed using MTS as described in Materials and methods. *denotes statistical significance with p-value ≤ 0.05.