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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Leuk Res. 2015 Jul 2;39(10):1071–1078. doi: 10.1016/j.leukres.2015.06.009

Fig. 5.

Fig. 5

The effect of 4-HPR on ceramide metabolism and viability in K562 and Molt-3 human leukemia cell lines. A. Effect of 4-HPR on ceramide generation and viability in K-562 cells. Ceramide generation was evaluated using [3H]palmitic acid as described in Materials and methods and in legend to Fig. 1. B. Effect of 4-HPR and C6-ceramide on K-562 cell viability. Cells were exposed for 72 h at the concentrations indicated; viability was by MTS. C. Effect of 4-HPR on ceramide metabolism in Molt-3 cells. 3H-cearmide, GC, and SM metabolism were evaluated using [3H]palmitic acid radiolabeling as detailed in Materials and methods. D. Effect of 4-HPR and C6-ceramide on Molt-3 cell viability. Cells seeded in 96-well plates were exposed to the agents shown at the indicated concentrations for 72 h after which viability was measured using MTS assays.