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. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Dev Cell. 2015 Aug 6;34(5):544–554. doi: 10.1016/j.devcel.2015.06.024

Figure 1. Axud1 is a marker of the premigratory neural crest in chick embryos.

Figure 1

All embryos are shown from a dorsal aspect. (A-B) In situ hybridization showed Axud1 expression in the Hensen’s node (A) and the paraxial mesoderm (B) in gastrulating chick embryos. (C-E) At neurula stages, Axud1 transcripts were detected in the dorsal neural folds (NFs) as they elevate and converge to form the neural tube. (F-G) Cross sections of neurula stage embryos showed specific expression of Axud1 in the dorsal neural tube. (H) At later stages, Axud1 was expressed in the early migrating neural crest (NC) and other structures targeted by Wnt signaling such as the mesonephros (MN) and the first presumptive somite. (I-F) Double in situ hybridization for Axud1 (I) and FoxD3 (J) revealed co-expression of both genes in the premigratory (K) and early migrating cranial neural crest (L). (M) Onset of FoxD3 expression was observed at HH8 within the Axud1 expression domain. (N) Expression of Axud1 in neural crest progenitors initiates after the establishment of the neural plate border but before neural crest gene expression, making it a likely candidate regulator in neural crest formation. HH: Hamburger and Hamilton developmental stages. HH: Hensen’s node, MN: Mesonephros, NF: Neural folds, NC: Neural crest, PM: Paraxial mesoderm, R: Rhombomere, SS: Somites, VNC: Vagal neural crest.