Figure 4.

Pretreatment with 5 μM DT3 or TRFRB preserved the cellular bioenergetics and mitochondrial respiration of H9c2 cells following H₂O₂ injury. Treatment of both DT3 and TRFRB increased basal oxygen consumption rate (OCR) as well as maximum OCR in H9c2 rat heart cardiomyocytes (a). Treatment with 5 μM of DT3 or TRFRB prior to 50 (b) or 100 μM H₂O₂ (c) resulted in complete protection and restored the mitochondrial respiration. Each data point represents mean ± SEM of n = 8–16 wells from 2 separate experiments. ^∗ p < 0.01 as compared to control, ^# p < 0.001 as compared to H₂O₂ treated group.