Table 3.
Agglutination reaction strengths of Immucor human polyclonal Anti-Fya and Anti-Fyb used to detect Duffy-like antigens on the surface of Dog Erythrocyte Antigen (DEA) 1+ and DEA 1− canine RBC that were either treated with papain (P) or left untreated (U). Gamma-clone Anti-Human Globulin (anti-IgG; polyspecific) was added for the reaction to occur.
| DEA 1+ | Anti-Fya | Anti-Fyb | |||
|---|---|---|---|---|---|
| Untreated | Papain | Untreated | Papain | ||
| A0 | 4+ | 0 | 2+ | 4+ | 3+ |
| A1 | 4+ | 0 | 1+ | 4+ | 4+ |
| A2 | 4+ | 3+ | 3+ | 4+ | 3+ |
| A3 | 3+ | 0 | 2+ | 4+ | 4+ |
| A4 | 3+ | 2+ | 3+ | 2+ | 3+ |
| A5 | 3+ | 0 | 1+ | 3+ | 3+ |
| A6 | 3+ | 3+ | 3+ | 2+ | 3+ |
| DEA 1− | |||||
| B1 | 0 | 2+ | 3+ | 3+ | 3+ |
| B2 | 0 | 3+ | 3+ | 2+ | 3+ |
| B3 | 0 | 3+ | 4+ | 3+ | 3+ |
| B4 | 0 | 2+ | 3+ | 0 | 4+ |
| B5 | 0 | 0+ | 1+ | 0 | 3+ |
| B6 | 0 | 3+ | 3+ | 0 | 3+ |
| B7 | 0 | 3+ | 2+ | 0 | 2+ |