Skip to main content
. Author manuscript; available in PMC: 2016 Sep 14.
Published in final edited form as: Dev Cell. 2015 Aug 27;34(5):592–607. doi: 10.1016/j.devcel.2015.07.014

Figure 8. Context-specific Regulation of Notch Signaling.

Figure 8

(A–B) Multiscale model of a Notch-mediated fate choice between ABala and ABara. (A) Notch signaling (N) induces the ABara fate. Color-coded trees below represent tissue marker expression patterns in the corresponding sublineages. (B) Gene regulatory networks that regulate cell fate choices between ABala and ABara. The network required for the ABara fate is shown in the red box, which contains known genes in the Notch pathway (stars). The network required for the ABala fate is shown in the blue box. It is further divided into 3 modules based on network connectivity. See also Figure S6.

(C) Differentiation of the ABala lineage for different genotypes. Expression of PHA-4 (red) was used to assay lineage differentiation. For each genotype a micrograph of embryo at the terminal stage is shown on the left (green labels all cells and red labels PHA-4 expressing cells) and PHA-4 expression pattern in the ABala lineage is shown on the right. Number shows the penetrance of each phenotype.

(D) Differentiation of the ABara lineage for different genotypes.

(E) Schematic representation of the CAF-1 complex.

(F) Quantification of ref-1::mCherry expression in ABala and ABarp lineages in the wild type and rba-1(RNAi). Each bar represents an embryo assayed. Expression level was averaged for ABala8 and ABarp8 cells in each embryo. P-value was calculated by T-test.

(G) Summary of genetic epistasis.