Figure 4. Tumoral expression of IL-36γ shaped the immunogenic tumor microenvironment.

From (A) to (G): 1×10⁵ B16-vector or B16-IL-36γ cells were injected i.d. into B6 mice. On day 24, tumors were resected and processed to generate a single cell suspension. (A) Percentages of CD45⁺ cells in tumor cell suspension. (B) Representative flow cytometric plots and percentages of CD8⁺ T or NK1.1⁺ cells within the gated CD45⁺ population in tumors. (C) Representative flow cytometric plots and percentages of γδ T or B cells within CD45⁺ population in the tumor microenvironment. (D) Percentages of the CD4⁺ T cells within the CD45⁺ TIL and percentages of CD4⁺Foxp3⁺ population within the CD4⁺ TIL. (E) Representative flow cytometric analysis of CD11b⁺ sub-populations and percentages of CD11b⁺ subpopulations of CD45⁺ immune cells and percentages of MHC class II expression on three CD11b⁺ sub-populations. Data (mean ± SEM) are average four independent experiments. * p < 0.05 and **p < 0.01 by two-tailed unpaired Student’s t-test. See also Figure S4.