Fig. 2.

Changes in iP and tZ concentrations and the expression of 38 cytokinin-related genes in developing Shiraz grape berries. a Changes in TSS, iP and tZ concentrations in field-grown Shiraz berries during the 2010/2011 season. All data represent means (n = 3) ± SE. “v” indicates veraison as determined by the last time point before a significant increase (p <0.05) in TSS levels was recorded. The asterisk marks the start of a significant increase in iP concentrations (p <0.05). FW, fresh weight. b Heat map showing changes in transcript levels of cytokinin-related genes expressed in berries as determined by qRT-PCR. In order to adjust for differences in absolute copy numbers between the genes, the mean (n = 3) expression values for each transcript were normalized by dividing by the maximum copy number obtained from the berry developmental series, making all values fall between 0 and 1. Each column represents a time point after flowering, each row represents a gene of interest. Hierarchical clustering was used to group genes with similar expression profiles. Copy numbers for the 29 genes expressed at more than two time points and statistical analyses of the data are given in Additional file 5