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. Author manuscript; available in PMC: 2016 Sep 16.
Published in final edited form as: ACS Chem Neurosci. 2015 Jul 22;6(9):1637–1648. doi: 10.1021/acschemneuro.5b00165

Figure 6.

Figure 6

qRT-PCR validation of RNA-Seq data in C57Bl/6 mice. (A) Total RNA was prepared for mRNA quantification by standard qRT-PCR. Tubulin was used as the internal control. The data were expressed as fold change versus nondiabetic mice for each gene. *p < 0.05 vs Veh + Veh; ^p < 0.05 vs STZ + Veh; n = 3. (B) Fold change versus nondiabetic mice using the normalized FPKM values from RNA-Seq