Table 3.
Results of fermentation experiments with 2 % glucose and addition of BQ or DMBQ
Compound (g/l) | Glucose start (g/l) | Glucose at time point t (g/l) | Glucose consumed at time point t (g/l) | YEtOH on consumed sugara | BEYb | QcEtOH | Time point t (h) |
---|---|---|---|---|---|---|---|
Experimental series A | |||||||
BQ | |||||||
0.02 | 19.5 ± 1.9 | 17.2 ± 1.7 | 2.4 ± 0.2 | 0.25 ± 0.03 | 0.03 ± 0.01 | 0.05 ± 0.01 | 12 |
0.20 | 19.5 ± 1.9 | 17.4 ± 1.7 | 2.2 ± 0.2 | 0.26 ± 0.03 | 0.03 ± 0.01 | 0.05 ± 0.01 | 12 |
1.00 | 19.5 ± 1.9 | 17.1 ± 1.7 | 2.5 ± 0.2 | 0.21 ± 0.02 | 0.03 ± 0.01 | 0.04 ± 0.01 | 12 |
DMBQ | |||||||
0.02 | 19.5 ± 1.9 | 2.3 ± 0.2 | 17.6 ± 1.7 | 0.41 ± 0.04 | 0.36 ± 0.04 | 0.59 ± 0.06 | 12 |
0.20 | 19.5 ± 1.9 | 17.4 ± 1.7 | 2.2 ± 0.2 | 0.22 ± 0.02 | 0.02 ± 0.01 | 0.04 ± 0.01 | 12 |
1.00 | 19.5 ± 1.9 | 16.2 ± 1.6 | 3.4 ± 0.3 | 0.13 ± 0.01 | 0.02 ± 0.01 | 0.04 ± 0.01 | 12 |
Reference | 19.5 ± 1.9 | 2.7 ± 0.3 | 17.3 ± 1.7 | 0.50 ± 0.05 | 0.43 ± 0.04 | 0.70 ± 0.07 | 12 |
Experimental series B | |||||||
BQ | |||||||
0.002 | 19.5 ± 1.9 | 2.7 ± 0.3 | 16.9 ± 1.7 | 0.50 ± 0.05 | 0.43 ± 0.04 | 0.35 ± 0.04 | 24 |
0.005 | 19.5 ± 1.9 | 2.7 ± 0.3 | 16.9 ± 1.7 | 0.49 ± 0.05 | 0.42 ± 0.04 | 0.23 ± 0.02 | 36 |
0.010 | 19.5 ± 1.9 | 2.6 ± 0.3 | 16.9 ± 1.7 | 0.49 ± 0.05 | 0.42 ± 0.04 | 0.23 ± 0.02 | 36 |
0.015 | 19.5 ± 1.9 | 2.7 ± 0.3 | 16.9 ± 1.7 | 0.48 ± 0.05 | 0.41 ± 0.04 | 0.17 ± 0.02 | 48 |
0.020 | 19.5 ± 1.9 | 17.5 ± 1.8 | 2.1 ± 0.2 | 0.27 ± 0.03 | 0.03 ± 0.01 | 0.03 ± 0.00 | 48 |
Reference | 19.5 ± 1.9 | 1.5 ± 0.2 | 18.0 ± 1.8 | 0.50 ± 0.05 | 0.46 ± 0.05 | 0.75 ± 0.08 | 12 |
The table shows the values at start and at a specific time point in the fermentation experiments. The relative standard deviation of the method that was used for monosaccharide and ethanol analysis was estimated to ≤10 %
ag EtOH/g consumed glucose
bBalanced ethanol yield; g EtOH/g glucose prior to fermentation
cVolumetric ethanol productivity; g EtOH × l−1 × h−1