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. 2015 Sep 16;10(9):e0138377. doi: 10.1371/journal.pone.0138377

Fig 5. Effect of proapoptotic BCL-2 family protein expression on the sensitivity to Ara-C, Nutlin-3a (N3a), KPT-330 (KPT) and ABT-199 (ABT).

Fig 5

(A) Immunoblot of BCL-2 family proteins in OCI-AML3 cells with control shRNA (ShC) or MCL-1 specific shRNA (ShMCL1). (B) Specific apoptosis induced by Nutlin-3a (2.5 or 5.0 μM), KPT-330 (500 or 1000 nM), Ara-C (2.5 or 5.0 μM) or ABT-199 (250 nM) in MCL-1-downregulated OCI-AML3 cells. ***P < 0.001 (C) Immunoblot of BCL-2 anti-apoptotic family proteins in HL-60 cells with overexpression of BCL-XL or BCL-2. (D) Specific apoptosis induced by Nutlin-3a (10 μM), KPT-330 (1000 nM), Ara-C (2.5 or 5.0 μM) or ABT-199 (250 nM) in BCL-XL- or BCL-2- overexpressing HL-60 cells. (E) Specific apoptosis induced by the combination of ABT-199 and Ara-C in KG-1 cells at 48 hours (left). Combination index was calculated by using CalcuSyn software (lower left). Annexin V- and PI-negative cells were counted as live cell number (right). (F) BH3 profiling of OCI-AML3 cells with control shRNA (ShC) or MCL-1 specific shRNA (ShMCL1), and HL-60 cells with control vector, overexpression of BCL-XL or BCL-2. The results are expressed as the mean ± SD. *P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001.