Strain |
E. coli BT340 |
DH5α/pCP20 |
[45] |
E. coli BW25113 |
K-12 derivative: ΔaraBAD, ΔrhaBAD
|
[46] |
E. coli DH5α |
For cloning and propagation of host strain, deoR recA1 endA1 hsdR17(rk-, mk
+) phoA supE44 thi-1 gyrA96 relA1 λ
−
|
Invitrogen, USA |
E. coli ET12567 |
dam-13::Tn9
dcm-6
hsdM
hsdS
|
[47] |
S. coelicolor M1152 |
Derivative of S. coelicolor M145, Δact Δred Δcpk Δcda
rpoB(C1298T) |
[28] |
S. coelicolor M1314 |
S. coelicolor M1152 Δsco7221
|
This study |
S. coelicolor M1316 |
S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671
|
This study |
S. coelicolor M1317 |
S. coelicolor M1152 Δsco7221 Δsco7669-7670-7671 Δsco1206-1207-1208::(oriT-aac(3)IV) |
This study |
Plasmid |
pGEM®-T Easy |
E. coli vector for cloning PCR product, ampr
|
Promega, USA |
pIJ86 |
ermEp*, aac(3)IV, oriT (RK2), ori (pIJ101), ori (pUC18) |
[48] |
pIJ773 |
pBluescript KS (+), aac(3)IV, oriT (RK2), FRT sites |
[35] |
pIJ790 |
λ-RED (gam, bet, exo), cat, araC, rep101
ts
|
[35] |
pIJ10701 |
pBluescript KS (+), hyg, oriT (RK2), bla
|
[35] |
pIJ12477 |
pIJ86, neo
|
[49] |
pIJ13000 |
St2H12 ∆sco7221::(oriT-aac(3)IV) |
This study |
pIJ13001 |
St4C2 ∆sco7669-7670-7671::(oriT-aac(3)IV) |
This study |
pIJ13002 |
2StG58 ∆sco1206-1207-1208::(oriT-aac(3)IV) |
This study |
pIJ13003 |
St2H12 ∆sco7221
|
This study |
pIJ13004 |
pIJ13003::(oriT-hyg) |
This study |
pIJ13005 |
St4C2 ∆sco7669-7606-7671
|
This study |
pIJ13006 |
pIJ13005::(oriT-hyg) |
This study |
pIJ13007 |
2StG58 ∆sco1206-1207-1208
|
This study |
pIJ13008 |
pIJ13007::(oriT-hyg) |
This study |
pUZ8002 |
Kanr
/tra, neo, RP4
|
[50] |
Cosmid |
2StG58 |
SuperCos1 containing sco1206-1207-1208
|
John Innes Centre, UK |
St2H12 |
SuperCos1 containing sco7221
|
John Innes Centre, UK |
St4C2 |
SuperCos1 containing sco7669-7670-7671
|
John Innes Centre, UK |
Sv3G11 |
SuperCos1 containing sven5367
|
John Innes Centre, UK |
SuperCos1 |
neo, bla
|
Agilent Technology, USA |