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. 2015 Sep 17;16:23. doi: 10.1186/s12860-015-0069-1

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© Sebastian et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Myotube formation was accompanied by the observation of lateral striations indicative of sarcomeric structures. a Myotubes at 4d, 5d, 9d (DM1) and 42d (DM1) of differentiation were immunostained for the presence of sarcomeric α-actin (Sigma A2172, 1:500 dilution). b Sarcomeric lateral striations were visible in H&E stained myotubes differentiated for 5d, derived from P3 and P5 longissimus dorsi myoblasts. Bar = 50 μm for all panels