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. 2015 Sep 16;6(1):177. doi: 10.1186/s13287-015-0168-0

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© Roubeix et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MSCs transplantation improves RGCs survival. Whole flat-mounted retina immunolabeled with brain-specific homeobox/POU domain protein 3A (Brn3a) antibody. Representative images (×200) of the peripheral or central area showing immunopositive staining of RGCs in noncauterized (Control) and cauterized eyes injected with MEM (EVC + MEM) or MSCs (EVC + MSCs) a Quantification of central b and peripheral c RGC densities per retina using automated nuclei counting in the control group (n = 5), EVC + MEM group (n = 5), and EVC + MSCs group (n = 5). The average RGCs density was obtained from eight peripheral and central images per retina and per area. Data expressed as mean ± standard error of the mean. *p <0.05 vs. control, #p <0.05 vs. EVC + MSCs. EVC episcleral vein cauterization, MEM minimum essential medium, MSCs mesenchymal stem cells