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. 2015 Sep 16;6(1):177. doi: 10.1186/s13287-015-0168-0

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© Roubeix et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — MSC-CM induces TM primary cell survival and contractility. Effect of MSC-CM on human trabecular meshwork (hTM) cell survival and contractility with or without transforming growth factor beta 2 (TGF-β ₂). hTM cell viability assessed by ATP quantification following 24 hours of increasing BAC concentration exposure with or without MSC-CM a. Immunoblot of phospho-Akt (p-Akt), ± MSC-CM, and phospho-myosin (p-MLC), ± MSC-CM and ± TGF-β_2, protein expression using WB in hTM cells was performed b, c. The relative density of protein bands were quantified, and the ratios of phosphorylated protein to actin were calculated (n = 4) b, c. *p <0.05 and ***p <0.001 vs. control culture condition, #p <0.01 vs. TGF-β₂ condition. MSC-CM conditioned medium of mesenchymal stem cells