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. 2015 Sep 17;17:128. doi: 10.1186/s13058-015-0630-z

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© Elsarraj et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — BCL9-KD decreased proliferative, migratory and invasive activity of DCIS cell lines in vitro. a Western blot analysis using anti-BCL9 antibody and anti-β-actin as a loading control (top), and (b) MTS assays of non-transduced (NT), scrambled control (control) and BCL9-KD in DCIS.COM (left panels) and SUM225 (right panels). Bar graphs represent mean absorbance at 490 nm normalized to NT ± standard error of the mean (SEM) (n = 3, * p <0.05). c, d Representative images of the migration and invasion assays. Bar graphs represent percent area of cells migrated (top) and invaded (bottom) under the membrane after 24 h for DCIS.COM and 96 h for SUM225. Invasion and migration were determined by ImageJ analysis of microscopic images per sample, the data are mean values normalized to NT ± SEM (n = 3, * p <0.05)