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. 2015 Sep 17;17:128. doi: 10.1186/s13058-015-0630-z

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© Elsarraj et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — BCL9-KD inhibits invasion in DCIS cell line mouse intraductal xenograft model (MIND) xenografts. a Immunofluorescent (IF) staining of BCL9 (red), K5/K19 (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue) in DCIS.COM (left) and SUM225 (right), control and BCL9-KD MIND xenografts at 10 and 14 weeks post-intraductal injection, respectively. b IF staining of K5 (red), smooth muscle actin (SMA) (green), and DAPI in DCIS.COM cells demonstrating how distance of invasion was measured. Scale bars = 50 μm, × 40 magnification. c Bar graphs represent the maximum distance of invasion and number of invasive lesions in control and BCL9-KD for DCIS.COM and SUM225 MIND xenografts. Data represent the mean ± standard error of the mean (n = 4, *p <0.05)