Fig. 3.

Activated CD45RO⁺ Tregs from sarcoidosis patients highly express CD95. The proportions of CD45RO and CD95 expressing Th cells and Tregs were determined in PB of HCs and SRC patients. a Representative flow cytometry analysis of an HC and SRC patient to determine the proportions CD45RO⁺, CD95^high and double positive Tregs (gated on CD3⁺CD4⁺CD25^int-highCD127^low.). b Proportions of CD45RO⁺ and CD95^high Th cells and Tregs. c Correlation between proportions CD45RO⁺ Tregs and CD95^high Tregs in PB. Open dots represent HC Tregs and closed dots represent SRC Tregs. d Mean fluorescence intensity of CD95 on CD45RA⁺ and CD45RO⁺ Tregs. Statistics: Horizontal lines indicate the median and significance was determined using a Mann–Whitney U test, * p < 0.05. Correlation was analysed using Spearman’s rank-order correlation test. Regression line with R and p-value are shown in the plot. Th T helper, CD95 Fas; death receptor, PB peripheral blood, HC healthy control, SRC sarcoidosis