Fig. 2.

SIS3 and Src-deficient mice inhibited hyperoxia-augmented lung stretch-mediated oxidative stress. a A Western blot was performed using an antibody that recognizes NOX2 expression and an antibody that recognizes GAPDH expression from the lungs of nonventilated control mice and those subjected to a tidal volume of 30 mL/kg for 1 h with room air or hyperoxia. Arbitrary units were expressed as the ratio of NOX2 to GAPDH (n = 5 per group). b NADP⁺-to-NADPH ratio, c MDA and d total antioxidant capacity were from the lungs of nonventilated control mice and those subjected to a tidal volume of 30 mL/kg for 4 h with room air or hyperoxia (n = 5 per group). SIS3 2-mg/kg was given intraperitoneally 1 h before ventilation. *P < 0.05 versus the nonventilated control mice with room air; † P < 0.05 versus all other groups. GAPDH = glyceraldehydes-phosphate dehydrogenase; MDA = malondialdehyde; NADP⁺ = nicotinamide adenine dinucleotide phosphate; NADPH = reduced NADP⁺; NOX2 = NADPH oxidase 2