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Effects of Δxrn1 and correction of the mutant on the stability of selected mRNAs. (A) 4TU RNA from wt, Δxrn1, and Δxrn1 xrn1[Barr] strains were analyzed by pulse–chase for arg-2, eif5, eif4a3, erf1, and cox-5 mRNAs using the procedures described in Figure 2. (B) Levels of mRNA in total RNA from wt, Δxrn1, and Δxrn1 xrn1[Barr] strains was quantified by RT–qPCR and mRNA levels were normalized to the level of 25S rRNA.
