Figure 1.

Testing for Nup160-mediated lethality in hybrid males between D. melanogaster and the three D. simulans clade species. D. melanogaster y w Df(1)Hmr v; Nup160⁻/CyO females were crossed to males from multiple isofemale lines of D. simulans (n = 11), D. sechellia (n = 6), and D. mauritiana (n = 9). For each, sex chromosome and second chromosome genotypes are shown (third and fourth chromosomes not shown), with D. melanogaster = white and D. simulans, D. sechellia, or D. mauritiana = gray.