Table 1. Nup160-mediated hybrid lethality is fixed in D. simulans and D. sechellia but absent in D. mauritiana.

	Hybrid females			Hybrid males
Male parenta	Nup160−	CyO	Relative viability	Nup160−	CyO	Relative viability	Percent rescue
D. simulans
sim 005	24	47	0.51**	0	20	0.00**	42.6
sim 007	32	50	0.64*	0	21	0.00**	42.0
sun 017	34	35	0.97	0	15	0.00**	42.9
sim 019	50	59	0.85	0	18	0.00**	30.5
sim 026	34	44	0.77	0	14	0.00**	31.8
sim 036	59	49	1.20	0	21	0.00**	42.9
sim 049	14	27	0.52*	0	14	0.00**	51.9
sim 058	21	33	0.64	0	16	0.00**	48.5
sim 065	37	46	0.80	0	20	0.00**	43.5
sim 224	48	77	0.62**	0	32	0.00**	41.6
sim w501	69	83	0.83	0	27	0.00**	32.5
Total	422	550	0.77**	0	218	0.00**	39.6
D. sechellia
sec Robertson	52	65	0.80	3	31	0.10**	47.7
sec Praslin	44	56	0.79	0	18	0.00**	32.1
sec w	39	33	1.18	1	15	0.07**	45.5
sec sy007	45	57	0.79	2	33	0.06**	57.9
sec sy034	46	88	0.52**	1	30	0.03**	34.1
sec iso81	33	63	0.52**	1	16	0.06**	25.4
Total	259	362	0.72**	8	143	0.06**	39.5
D. mauritiana
mau iso105	49	50	0.98	17	15	1.13	30.0
mau iso152	58	62	0.94	30	24	1.25	38.7
mau R11	70	53	1.32	22	16	1.38	30.2
mau R23	41	36	1.14	8	19	0.42*	52.8
mau R35	39	43	0.91	13	19	0.68	44.2
mau R45	47	45	1.04	17	11	1.55	24.4
mau R48	53	49	1.08	23	18	1.28	36.7
mau R57	30	30	1.00	13	16	0.81	53.3
mau w	215	205	1.05	82	73	1.12	35.6
Total	602	573	1.05	225	211	1.07	36.8

^*P < 0.05, **P < 0.01, χ²-tests.

^aFor all crosses, D. melanogaster y w Df(1)Hmr v; Nup160⁻/CyO females were crossed to D. simulans, D. sechellia, or D. mauritiana males.