Figure 2.

Visualization of transfer and localization of G-SP. Transgenic males expressing G-SP were mated with wild-type females. (A) Mated pair soon after the beginning of copulation and (B) 1 min later. (C) Dissected female reproductive tracts soon after the end of copulation. (D) Confocal image of reproductive organs 2 hr after the end of copulation. The GFP signal was detected in the sperm storage organs (spermathecae and seminal receptacles). (E) Distribution of G-SP in the females mated with X/O males. Strong GFP signal is from the uterus, while no or little signal was detected in seminal receptacle, spermatheca, and oviduct. (F) Binding of G-SP to the sperm. Sperm from the females mated with transgenic males expressing indicated fusion protein. Confocal (top) and Nomarski images (bottom). Both N- and C-terminal sequences contribute to G-SP binding to sperm.