Figure 3.

Girdin is essential for sensory dendrite formation. (A and B) The development of sensory neuron in the thorax was monitored using confocal microscopy of GFP-tubulin. Starting from 30 hr APF, images were taken every 10 hr up to 90 hr APF in wild type (A) and girdin¹⁰¹ (B). Bar, 20 μm. (C) A single neuron was followed during development using live confocal microscopy of GFP-tubulin. Bar, 10 μm. A 488-nm laser with 5% power was applied for GFP fluorescent excitation; emission photons were collected from the wavelength 490–550 nm for the green and 620–750 nm for the red autofluoresce. The merge of this autofluorescence results in orange color. Ax, axon; CB, cell body; Den, dendrite. (D) Magnification of dashed-line box is at 80 hr APF in girdin¹⁰¹. Sensory outer segments labeled by GFP-tubulin are virtually absent in girdin¹⁰¹ (white arrow). Dendrites labeled by GFP-tubulin are absent in girdin¹⁰¹ (arrowhead). Green: GFP-tubulin. Red: cuticle autofluorescence. Bar, 5 μm. (E) Quantification of the percentage of normal neuron (having a dendrite with a cilium at the expected location for each stage), abnormally short or missing dendrites, or missing neurons in girdin¹⁰¹ over sensory organ development starting at 30 hr APF until 90 hr APF. Shown as mean ± SD; n = 250. (A–D) elav-Gal4;UAS-GFP-tubulin;MKRS/TM6B (control) and elav-Gal4;UAS-GFP-tubulin;girdin¹⁰¹ (101).