Table 3.
Sensitivity of freshly separated glioma xenograft cells to HSV infection
| Subpopulation | Sensitivity to HSV (LD50/PFU)a
|
D456 versus GBM12 P value | ||
|---|---|---|---|---|
| D456MG | GBM12 | UAB 1066 | ||
| HSV-1 all cells (range) | 1.0 ± 0.2 (0.8–1.4) | 7.8 ± 2.3 (4.6–9.9) | > 100 | 0.01 |
| CD133− (range) | 0.3 ± 0.1 (0.2–0.3) | 9.4 ± 2.1 (7.3–11.5) | > 100 | 0.01 |
| CD133+ (range) | 1.3 ± 0.2 (1.0–1.5) | 3.9 ± 1.0 (3.1–4.7) | > 100 | 0.02 |
| All cells vs. CD133+ | P = 0.33 | P = 0.2 | – | 0.01 |
| Δγ134.5b all cells (range) | 3.0 ± 2.4 (0.8–8.7) | 8.5 ± 4.7 (1.9–14.8) | > 100 | 0.1 |
| CD133− (range) | 1.9 ± 1.1 (0.6–3.4) | 4.4 ± 2.2 (2.0–7.5) | > 100 | 0.07 |
| CD133+ (range) | 3.4 ± 3.1 (0.9–7.9) | 5.6 ± 2.7 (2.8–9.9) | > 100 | 0.7 |
| All cells vs. CD133+ | P = 0.8 | P = 0.5 | – | |
a
Values are given in LD50/PFU based on titration of each virus in graded dilutions
b
γ134.5-deleted viruses used include G207, M002, R3659, C101