Video 2. Intravital TP-LSM images of the gp120 loaded cellular network in the IFC of the inguinal LN.
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DOI: 10.7554/eLife.06467.012
An image sequence of a 30 μm z-projection was acquired from a LysM-EGFP mouse, which had previously received by adoptive transfer both B cells, blue, and CD4 T cells, purple. Host endogenous neutrophils/monocytes, strong green signal, and stromal cells, weak green signal, can be seen on the basis of their expression of LysM-EGFP. Images were acquired for an hr from 1.5–2.5 hr after fluorescent gp120, white, injection near the tail base. GFP positive cells can be seen flowing in blood vessels in the IFC. CD169 antibody, red, delineated the SSMs. Second harmonic signal, blue, from collagen delineated LN capsule. Scale bar is 100 μm. Time counter shows hr:min:s.