Video 5. Intravital TP-LSM images of in vivo calcium response of a b12 B cell engaging a gp120 bearing IFC cell.
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DOI: 10.7554/eLife.06467.020
An image sequence of a 25 μm z-projection was acquired from the inguinal LN of a mouse, which has fluorescent gp120 (green) delineated IFC network cells. b12 HL B cells (blue) were labeled with calcium orange (red). Shaded circle indicates the interaction between b12 HL B cells and gp120 loaded IFC network cells. Images were acquired for 40 min beginning 41 min after fluorescent gp120 injection into tail base. Scale bar is 15 μm. Time counter shows hr:min:s.