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. 2015 Sep 16;10(9):e0137835. doi: 10.1371/journal.pone.0137835

Fig 5. CD205, Arginase1, Fizz1 and CD301 expression on infected spleen cells from IL12p40-deficient BALB/c mice.

Fig 5

IL12p40-/- STAT6+/+ BALB/c mice were injected i.n. with 2x107 CFU of mCherry-Br. The mice were sacrificed at 28 days post-infection and the spleens were collected and examined by immunohistofluorescence. for the expression of CD205 and M2 markers and co-localization with mCherry-Br. The left panels show the overall distribution of the Dec205-, Arg1-, Fizz1- and CD301-expressing cells in the spleen. The middle panels show mCherry-Br co-localization with negative cells for Dec205, Arg1, Fizz1 and CD301. The right panels present the immunofluorescence analysis of mCherry-Br co-localization with positive cells for Dec205, Arg1, Fizz1 and CD301. The panels are color-coded with the text for DAPI, phalloidin, the antigen examined or mCherry-Br. Scale bar = 200 and 20 μm, as indicated. r.p.: red pulp; w.p.: white pulp. Yellow arrowheads indicate the presence of bacteria. The data are representative of at least two independent experiments.