Table 3.
Steady-state GTP hydrolysis parameters for TBC-DEG, TBCC, and αβ-tubulin
| GTP hydrolysis reactions | Km (GTP) | kcat (min−1/μM) |
|---|---|---|
| 1 μM TBC-DEG | 400 ± 30 μM | 0.06 ± 0.01 |
| 1 μM TBCC:1 μM αβ-tub | 20 ± 15 μM | 0.00 ± 0.01 |
| 1 μM TBC-DEG:1 μM αβ-tub | 69 ± 12 μM | 0.40 ± 0.01 |
| 1 μM TBC-DEG:1 μM TBCC | 94 ± 10 μM | 0.77 ± 0.04 |
| 1 μM TBC-DEG:1 μM TBCC:1 μM αβ-tub | 99 ± 10 μM | 1.88 ± 0.03 |
| 1 μM TBC-DEG-Q73L:1 μM TBCC:1 μM αβ-tub | 371 ± 20 μM | 0.50 ± 0.05 |
| 1 μM TBC-DEG:1 μM TBCC:0.25 μM αβ-tub | 87 ± 5 μM | 1.10 ± 0.06 |
| 1 μM TBC-DEG:1 μM TBCC:0.5 μM αβ-tub | 112 ± 4 μM | 1.41 ± 0.05 |
| 1 μM TBC-DEG:1 μM TBCC:1.5 μM αβ-tub | 98 ± 8 μM | 2.38 ± 0.08 |
| 1 μM TBC-DEG:1 μM TBCC:3.0 μM αβ-tub | 88 ± 9 μM | 2.77 ± 0.11 |
| 1 μM TBC-DEG:0.12 μM TBCC:1 μM αβ-tub | 149 ± 7 μM | 1.53 ± 0.04 |
| 1 μM TBC-DEG:0.25 μM TBCC:1 μM αβ-tub | 129 ± 3 μM | 1.69 ± 0.02 |
| 1 μM TBC-DEG:0.50 μM TBCC:1 μM αβ-tub | 108 ± 5 μM | 1.74 ± 0.04 |
| 1 μM TBC-DEG:1 μM TBCC-R186A:1 μM αβ-tub | 40 ± 10 μM | 0.54 ± 0.04 |
| 1 μM TBC-DEG:1 μM TBCC-Δ233-245:1 μM αβ-tub | 35 ± 8 μM | 0.34 ± 0.03 |
| 1 μM TBC-DEG:1 μM TBCC-Cterm:1 μM αβ-tub | 56 ± 7 μM | 1.13 ± 0.07 |