Fig 3. Effects of alginate on cellular iron transport.

(A) Intracellular iron concentration decreases when RKO cells were incubated with iron-59 and alginate (0.3% w/v) compared to iron only control (B) Treatment of RKO cells with iron increases ferritin expression whilst co-incubation with alginate (0.3% w/v) significantly suppressed the iron mediated ferritin induction. All experiments were performed in triplicate with error bars representing +/- SEM and * denotes statistical significance at p < 0.05.