FIGURE 4.

24E9 mAb and 24E9 Fab inhibit IE binding to ICAM-1 under flow conditions. Inhibition of adhesion by 24E9 mAb and 24E9 Fab of 3D7 DC4⁺ (A) and BM57 DC4⁺ (B) to rICAM-1 coated onto Biochips. Abs were titrated at 0.1–10 μg/ml (24E9 mAb) and 0.001–1 μg/ml (24E9 Fab). Each condition was run in triplicate for a minimum of three independent experiments and expressed as average number bound per square millimeter compared with untreated controls. Mouse IgG and mouse IgG Fab fragments were added as controls. Statistical significance was determined via one-way ANOVA with Tukey’s multiple comparison test. **p < 0.05, ***p = 0.0001.