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. 2015 Aug 28;195(7):3273–3283. doi: 10.4049/jimmunol.1501404

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Copyright © 2015 The Authors

This is an open-access article distributed under the terms of the CC-BY 3.0 Unported license.

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FIGURE 5. — 24E9 mAb recognizes a conformation epitope on PFD1235w DBLβ3_D4. (A) Western blotting of PFD1235w DBLβ3_D4 (D4) and DBLβ3_D5 (D5). +DTT (reduced), −DTT (nonreduced). Lane 1, Prosieve protein marker (M) visualized by phosphorescent paint as dots. Lanes 2 and 3, DBLβ_D4 (±DTT). Lanes 4 and 5, DBLβ3_D5 (±DTT). Arrow shows nonreduced DBLβ3_D4 (lane 3) recognized by 24E9 mAb. (B) 24E9 mAb ELISA reactivity against reduced (+DTT) and nonreduced (−DTT) PFD1235w DBLβ3_D4. (C) AB01 mAb ELISA reactivity against reduced (+DTT) and nonreduced (−DTT) DBLγ of PFD1235w. Mean OD values are shown for three independent experiments. Error bars indicate SD.