Table II. Kinetic parameters derived from SPR experiments on 24E9 mAb interacting with mutant version of PFD1235w DBLβ3_D4.
Interaction | ka (×107 M−1 s−1) | kd (×10−5 s−1) | KD (pM) | Rmax (RU) | Model |
---|---|---|---|---|---|
DBLβ3_D4 wild type::24E9 mAb | 6.4 | 7.7 | 1.2 | 229.2 | One-site |
DBLβ3_D4_P2b_D5::24E9 mAb | 1.87 | 4.1 | 2.19 | 239.2 | One-site |
DBLβ3_D4_P3a_D5::24E9 mAb | 2.2 | 24 | 110 | 8.64 | One-site |