Table III. Experimental values derived from PFD1235w DBLβ3_D4::24E9 Fab SAXS experiments.
| Rgexp (nm) | Dmax (nm) | Vporod (nm3) | Mrexp (kDa) | Mrapp (kDa) | χ | |
|---|---|---|---|---|---|---|
| DBLβ3_D4 | 2.69 | 9.39 | 91.13 | 55 | 54.45 | 4.57 |
| DBLβ3_D4::24E9 Fab | 3.81 | 12.16 | 140.17 | 105 | 91.05 | 3.456 |
The experimental Rg (Rgexp) was determined using AutoRG (8), the Dmax was derived from GNOM (30), and the Porod volume (Vporod) was determined by using PRIMUS (29). The expected molecular mass (Mrexp) is shown for DBLβ3_D4 and the DBLβ3_D4::24E9 complex. The apparent molecular mass (Mrapp) was calculated from the volume excluded in the final DAMMIN (33) model divided by 2. The χ value represents the best fit of 20 low-resolution shape reconstructions using ab initio modeling.