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. 2015 Sep 18;16:24. doi: 10.1186/s12860-015-0070-8

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© Lee et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Acetylated tubulin mediated by intracellular sodium level regulates vesicle trafficking between endosomes. a DAOY cells were treated with AlexaFluor 488-EGF for 30 minutes a, b) or 60 minutes (C, D) in High Na⁺ or Low Na⁺ buffer. Immunofluorescence of early endosomes (EEA1) (A) and late endosomes (LAMP2) c are shown. The squares depict the areas of higher magnification. Scale bars represent 10 μm (low magnification) and 1 μm (high magnification). (B and D) The colocalization ratio was calculated using ImageJ and plotted as mean with standard errors.